mercredi 11 février 2015

The Right Kit For Solubility Screening

By Beryl Dalton


Protein aggregation affects their availability for certain functions. This calls for solubility screening to establish the formulations or conditions that will protect your proteins. The choice of kit will determine the comprehensiveness or intensity of the results you obtain. The protein samples are solubilized during testing depending on expectations.

Aggregation properties and capabilities are easily detected by using PH, salt and other additives. A label free form and a parallel form will give an idea about the conditions being examined. Testing does not require the use of centrifuges. The desired results are obtained through simple mixing and spinning of the agents being tested. This increases the range of circumstances that can give desired results.

The results obtained using the screening kits enable you to rescue reversibly aggregated protein samples. Your proteins will also not aggregate under stress. This makes them available for different tests and functions. You are provided with a dashboard with trends that make it easy to interpret results from the tests. The dashboard makes it easier to relate the results and make inferences.

Protein aggregation is an undesirable process that is attributed to a number of conditions. These conditions include vigorous stirring, elevated temperatures, addition of molecular binding partners or ligands and prolonged storage of protein. It is possible to avoid aggregation by making the right choices on stabilizing additives, PH and salt.

With a PH range between 3 and 10, the screening kit offer sufficient buffer. It also comes with solubility enhancers such as sugars, salts, amino acids, reducing agents and polyols, among others. The results are clear and precise which allows you to identify aggregating and non-aggregating proteins.

Working with this kit allows you to get over ninety formulations using a single label free experiment. You will use the other six as control to get positive and negative assessments. The expected results include soluble proteins going through the filters while aggregated ones do not. The filtration process will help you get these results.

Protein solubilization tests give the conditions under which your proteins will not aggregate. From such tests, it is observed that filtration processes yield proteins when they are in soluble form. They are available in aggregated form if the test does not show any traces of proteins.

The results of screening tell of the best conditions that the proteins will perform. It gives you an opportunity to isolate proteins that behave well for your intended purpose and those that cannot fit your intentions. With such results, you can scale up the use without fear since you have confirmed the results.

The benefits of using screening kits over other testing methods are numerous. Results obtained are clear and easy to interpret based on the information available in the field. The results leave no room for doubt. It takes as little as one hour to get your results. These results are certain and not based on trial and error.

The amount of protein you will require is substantially reduced. This allows it to be evenly matched to the target detection assay. This method is also convenient since it provides a lot of information in one test. The kit also allows you to use your assay of choice without being restricted to certain formulations.




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